Outstanding presentation, some really solid character work for the party members, and maybe some questionable execution on the My Wolf component.
Breaking down by rating criteria, spoilers from this point forward.
Implementation of Theme:
It's present. The "darkness" here is Matteo's struggle with self-doubt and lack of a sense of belonging, the first "light" to counter that darkness being Vito, with Matteo opening to a secondary "light" of forming bonds with Sunrise Fang given Vito's unknown status. I think there could have been a bit more finesse in handling the theme, both in further developing Vito and in getting the latter point across with more than the series of essentially 1-on-1 monologues from the other party members, but you got the point across.
Story:
While the initial character introductions could have been handled a bit more smoothly (took me a bit to figure out which character was the MC), I think the dynamics of the party (minus Matteo - I'll get to that) are really well done. Diego, Valentina, and Sesto all feel for the most part like developed characters with their own personalities and quirks, and while the latter are sometimes exaggerated for effect I think the humor really comes through for all three of them which really helps sell the bonds present in the party.
With that said, the characterizations of Vito and Matteo comparatively feel a bit sparse. For Vito, really the only things we know about him are: 1) good adventurer and 2) loves his boyfriend. We don't get much more for Matteo: 1) less good adventurer; 2) sadsack; 3) also loves his boyfriend. It's tough to evaluate this entirely since it seems like there's more to come in the story, but for such a central component of the narrative there could really be more meat on these characters. The line from Matteo contemplating Vito, "Will I ever get to see what he sees in me?" especially stands out since other than being kind of a bummer we don't get much about Matteo's personality, so my reaction was along the lines of, yeah, I don't really know what he sees in you either.
Also, Diego to Matteo: "You know, you can be quite funny at times, and I am not easily amused" - it's usually kind of a bad idea in my opinion to have a character tell another character they're funny. Either the character is actually funny and the humor is already coming through, so it's redundant, or the character is not actually funny and the line inadvertently calls attention to that. As the least funny member of the party, Matteo unfortunately falls into the latter category.
Quick note about the use of Italian in the story - I didn't have a problem following it, but I also don't know if I understand the point. Usually when a character slips into another language, it's to emphasize their uniqueness, i.e., "that character is the Italian one." However, in this story, all the places have Italian names and all the characters have Italian names, so the presumption is they're already speaking their own language and that everything is written in English for the audience's benefit. So here you have characters slipping into a language they all know while they're already speaking a language they all know.
Presentation:
It's good. Very good. I think you know it's good, but it bears repeating. There were moments when I wish there were some extra sprites here and there to convey emotion (Diego's line "Not once have you failed to add meaningful commentary in response to the current situation" is so devastatingly sarcastic that I wish there had been a proper visual to accompany it) but really the art, UI, music, "speaking" effects, all of it came together really well for me.
The "You Win!" screen after a battle in which I contributed precisely nothing was also hilarious to me.
Creativity:
I can't fault you here - this went well and beyond the expectation for what you could churn out in the timeframe of the jam and this entry oozes passion and effort. Just wish things had been a hint tighter with the main character and the love interest. Will keep an eye out for this one to see where it goes.